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KMID : 0379520010170000293
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한국독성학회지
2001 Volume.17 No. 0 p.293 ~ p.297
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CB6F1-Tg rasH2 Mouse Carrying Human Prototype c-Ha-ras Gene As an Alternative Model For Carcinogenicity Testing For Pharmaceuticals
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USui T.
Urano K.
Suzuki S.
Hioki K.
Maruyama Ch.
Tomisawa M.
Ohnishi Y.
Suemizu H.
Yamamoto S.
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Abstract
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The international pharmaceutical and regulatory communities had been recognizing the limited utility of conventional rodent carcinogenicity study particularly on the second species, mouse, after intense investigation of carcinogenicity data base worldwide, and a new scheme for carcinogenicity testing for pharmaceuticals was proposed at the Expert Working Group on Safety in the International Conference on Harmonization (ICH) in 1996. CB6F 1-Tg rasH2 mouse carrying human prototype c-Ha-ras gene with its own promoter/enhancer is one oj the new carcinogenicity assay model for human cancer risk assessment. Studies have been conducted since 1992 to validate the transgenic (Tg) mice for rapid carcinogenicity test-ing, short term (26 weeks) studies with genotoxic (by Salmonella), non-genotoxic carcinogens, genotoxic non-carcinogens, non-genotoxic non-carcinogens revealed relatively high concordance oj the response of the Tg mouse with classical bioassay across classes of carcinogenic agents. Mechanistic basis for carcinogensis in the model are being elucidated in terms of the role of overexpression and/or point mutation of the transgene. This report review the initial studies of validation of the model and preliminary results of on-going ILSI HESI ACT project will be presented.
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KEYWORD
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rasH2 mouse, CB6F1-TgrasH2 mouse, Human c-Ha ras proto-oncogene, Short-term carcinogenicity test, Transgenic mouse, Rodent carcinogen, Chemical carcinogenesis
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